Department für Physik - Lehrstuhl Prof. Rädler
1. Development of a Quantitative Single Cell Cytolysis Assay
Natural killer (NK) cells, a subtype of cytotoxic lymphocytes, play a crucial role in immunity. They recognize infected and cancerous cells, form with them a specific one-to-one immune synapse and eventually eliminate them through the perforin/granzyme pathway. This innate mechanism can be manipulated as a potent cancer therapy using antibodies or recombinant proteins that target both cell types of interest. Our aim is the development of single-cell platforms to uncover the special characteristics of the cell-mediated cytolysis.
2. Development of a single-cell ELISA assay
3. Label-free monitoring of individual cell timing after induction of apoptosis using micro-trenches arrays
Chip-based platform for dynamic analysis of NK cell cytolysis mediated by a triplebody. Elisavet I. Chatzopoulou, Claudia C. Roskopf, Farzad Sekhavati, Todd A. Braciak, Nadja C. Fenn, Karl-Peter Hopfner, Fuat S. Oduncu, Georg H. Fey and Joachim O. Rädler. Analyst, 2016, DOI: 10.1039/C5AN02585K