Soft Condensed Matter Group
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Versuch R3b: Super-resolution microscopy and DNA nanotechnology

Location

Jungmann Lab
MPI of Biochemistry
Am Klopferspitz 18
82152 Martinsried

Supervisor

Alexander Auer
Tel.: 089 8578 3413
aauer@biochem.mpg.de

Description

In this one-day lab course you will combine DNA nanotechnlogy e.g. DNA origami with super-resolution microscopy. It starts with designing DNA origami structures for a DNA-PAINT experiment using our lab software 'Picasso'. Afterwards, the DNA origami structures are synthesized and imaged with a custom TIRF microscope. The course constitutes an intensive training in a number of DNA nanotechnology and single molecule flouresence techniques including DNA origami folding, gel electrophoresis, slide preperation, super-resolution imaging, and image analysis and post-processing.

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